Alcohol or soap wash
The most reliable field method, but it kills the sampled bees. It is useful when the result matters.
Turn a mite wash, sugar roll or CO2 sample into mites per 100 bees, then compare the result with a seasonal treatment threshold.
Treatment thresholds change through the season. Choose the phase that best matches the colony.
Mite levels are usually shown as mites per 100 adult bees. Treat the threshold as a ceiling, not a goal.
| Colony phase | Typical timing | Treat at |
|---|---|---|
| Dormant | Winter cluster, little brood | 1% |
| Population increase | Spring build-up, before main flow | 1% |
| Peak population | Summer peak, honey supers may be on | 2% |
| Population decrease | Post-harvest, winter bees being raised | 2% |
If a colony is approaching the threshold, do not wait for mites to explode. It is much easier to keep mite pressure low than to rescue a colony once viruses and winter-bee damage are already underway.
The most reliable field method, but it kills the sampled bees. It is useful when the result matters.
Non-lethal, but less consistent. A borderline result is worth checking with a wash.
Quick and non-lethal, but recovery varies. Treat the number as a useful indicator rather than a perfect count.
A common working line is around 1% during dormant and spring build-up phases, and around 2% during peak and declining population phases. Local guidance may vary.
Divide mites counted by the number of bees sampled, then multiply by 100. Six mites in 300 bees is 2%.
An alcohol or detergent wash is usually more reliable, while sugar rolls are non-lethal but can miss mites. Confirm borderline sugar roll counts with a wash.
Sample nurse bees from open brood, after checking the queen is not on the frame. Bees from honey frames can give a falsely low count.
No. Mites can build quickly. Sample again through the active season and check again after treatment to confirm it worked.